What Is LH (Luteinising Hormone)?

Luteinising hormone (LH) is produced by the pituitary gland — a small gland at the base of the brain that acts as the control centre for your reproductive hormones. LH is present in your bloodstream throughout your cycle, sitting at a low baseline level while your body prepares for ovulation.

Its role: alongside FSH (follicle-stimulating hormone), LH encourages follicles in your ovaries to develop and mature. But it has one specific, dramatic job that happens once per cycle — triggering the release of the egg.

What Is the LH Surge?

About 24–36 hours before ovulation, something shifts. Oestrogen — which has been rising steadily as the dominant follicle grows — reaches a peak that flips a switch in the hypothalamus. The pituitary responds with a massive release of LH: a spike that can be three to ten times higher than normal baseline levels.

This is the LH surge. It is the biological on-switch for ovulation. When the follicle is flooded with LH, it swells, ruptures, and releases the mature egg into the fallopian tube. The surge lasts roughly 24–48 hours before LH levels fall back down.

No LH surge = no ovulation. It is that direct a relationship.

When Does the LH Surge Happen?

The timing depends on your cycle length. Because ovulation consistently occurs approximately 14 days before your next period (not after your last), you can estimate when to expect the surge:

Cycle lengthEstimated ovulation dayEstimated LH surge
24 daysDay 10Days 8–9
28 daysDay 14Days 12–13
32 daysDay 18Days 16–17
35 daysDay 21Days 19–20

These are estimates — the actual day can shift based on stress, illness, travel, and your body’s individual variation. Our free ovulation calculator uses your cycle length to give you a personalised estimate of when your surge is most likely to fall, so you know when to begin testing.

How OPK Tests Work

OPK (ovulation predictor kit) test strips detect LH in your urine. They work on the same principle as a pregnancy test: a chemical reaction produces a visible line when a target hormone is present above a threshold.

How to read the result:

  • The test has two lines — a control line and a test line
  • The test line appears when LH is present in your urine
  • Negative: The test line is lighter than the control line
  • Positive: The test line is as dark as or darker than the control line
  • The positive reading means the LH surge is underway and ovulation is 24–36 hours away

A common confusion: a faint test line does not mean positive. LH is always present at low levels, so a faint line is normal background. You are looking for a test line that clearly matches or exceeds the darkness of the control.

Best Time of Day to Test

This matters more than most instructions acknowledge: do not use first morning urine for LH testing.

Here is why. The LH surge begins in the blood approximately 4 hours before it appears at detectable levels in urine. If you test first thing in the morning, you may be testing urine that was produced during the night — before the blood surge had time to transfer into the urinary system.

The best window is between 10am and 8pm. Mid-morning to mid-afternoon catches the surge most reliably.

Also: avoid drinking large amounts of fluid in the 2 hours before testing, as this dilutes LH concentration and can produce a false negative.

When to Start Testing

Start testing 3–5 days before your estimated ovulation date. This gives you enough lead time to catch the surge without testing for weeks. Use our ovulation calculator to identify your estimated ovulation date, then count back 4 days — that is when to start.

For a 28-day cycle, this typically means starting on day 10.

Test once daily at the same time. If you detect a positive, you can test again 12 hours later to confirm the surge is building — but for most purposes, one daily test is enough.

LH Surge and PCOS — A Note from Kanika

I want to be direct with you here because this is something I navigated myself, and I wish someone had explained it clearly at the start.

I have PCOS. When I first started using OPK strips to track ovulation, the results were genuinely confusing. I would get what looked like a positive on day 10 — then another on day 14 — then another on day 17. I had no idea which, if any, was a real surge. I remember feeling like I was doing something wrong.

I was not doing anything wrong. PCOS commonly causes elevated baseline LH levels throughout the cycle. This means the test line on an OPK can look positive — or near-positive — on days when ovulation is not actually happening. Standard OPK instructions do not mention this, which is genuinely frustrating.

What helped me:

Look for the peak, not just a positive. If you have been running a moderately dark test line for several days and suddenly it is significantly darker — noticeably, clearly the darkest it has been — that is almost certainly your true surge. The real surge tends to be a visible jump, not just a line crossing a threshold.

Combine OPKs with BBT. Basal body temperature rises 0.2–0.5°C after ovulation due to progesterone. Tracking this alongside OPKs confirms retrospectively whether the surge you detected was a real one. After a cycle or two of both methods, you start to understand your own pattern.

Consider quantitative LH monitoring. Some apps and digital OPK readers track the actual LH level numerically rather than just giving a positive or negative. These are more useful for PCOS because they let you see the actual peak rather than comparing lines.

If your OPK results are confusing you — multiple positives, no clear pattern, lines that seem to do nothing consistent — please know that this is an extremely common PCOS experience. You are not misreading the tests. The tests just were not designed with PCOS in mind.

LH Surge with Irregular Cycles

If your cycles are irregular, timing OPK testing becomes harder because you cannot reliably predict when the surge will fall. The approach:

  • Start testing from day 8–10 of every cycle, regardless of how long your cycles tend to be. Early ovulation is rare but possible, and missing the surge by starting too late is the most common pitfall.
  • Test daily without skipping — the surge can be brief, sometimes lasting fewer than 24 hours in some people.
  • Keep testing until positive or until your period arrives — with a long irregular cycle, this may mean testing for many days.

Our irregular period calculator can generate a window estimate from your recent cycle history, helping you narrow down the likely surge window even with unpredictable cycles.

What to Do After a Positive OPK

A positive OPK is your most actionable piece of cycle data. Here is what it tells you and what to do with it:

Ovulation will occur within 24–36 hours. The fertile window is open right now. The egg will be released soon, and sperm already present in the fallopian tubes will be in the right place to meet it.

If you are trying to conceive:

  • Have unprotected sex the day of the positive OPK
  • Have unprotected sex again the following day
  • The day before the positive is also fertile — if you have been tracking carefully and had sex then, that counts too

If you are tracking for cycle awareness:

  • Note the positive in your chart
  • Expect your period approximately 12–16 days later (this is your luteal phase length)
  • If your period arrives significantly earlier or later than expected, that is useful information about your luteal phase

No LH Surge Detected — What Might It Mean?

If you have tested daily at the right time and never got a positive, there are several possible explanations:

You missed the window. The LH surge can be brief — sometimes peaking and falling within 12–24 hours. If you tested at inconsistent times or skipped a day, you may have missed it.

The test was too diluted. Excess fluid intake before testing, or testing with very diluted urine, can lower LH concentration below the detectable threshold.

An anovulatory cycle. Some cycles simply do not produce ovulation — this is common and normal occasionally, particularly during stress, illness, significant weight changes, or in perimenopause. Anovulatory cycles happen to most people occasionally.

PCOS or another hormonal condition. As above, PCOS can affect LH patterns. Thyroid dysfunction and hyperprolactinaemia can also suppress or disrupt the LH surge.

If you have tested correctly for two or more cycles and consistently find no surge, raise it with your GP. A blood test for LH, FSH, and progesterone (ideally on day 21 of a 28-day cycle) can confirm whether ovulation is occurring. It is a simple test and worth asking for.